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AQUA-SOIL RESEARCH
 
Pot trial with Aqua-Soil applied to Pinus Patula and a Eucalyptus clone
Nitrogen Retention Test
Phosphate Retention Test
Potassium Retention Test
Water Retention Test after 30 Days
 
Research Archive
 
Pot Trial With Aqua-Soil applied to Pinus Patula and a Eucalyptus clone
 

INTRODUCTION

 
Pot trials had been conducted to simulate field planting of Pinus radiata. Application of Aqua-Soil stimulated height and root growth statistically significantly. (See previous report) Subsequently, new formulations of Aqua-Soil were prepared and similar pot trials were established to test their effects on Pinus patula and a Eucalyptus clone.

The results are summarized and briefly discussed below. Once again, the statistically significant growth responses indicated that Aqua-Soil could revolutionize tree establishment practices

 

MATERIALS AND METHODS

 
Apart from the control treatment (400 ml water), the following Aqua-Soil and water treatments were prepared and allowed to gel for 30 minutes:
  •  5 g Aqua-Soil (Forest) in 200 ml, 300 ml, 400 ml and 500 ml water;
  • 10 g Aqua-Soil (Forest) in 300 ml water. 
  •  3 g Aqua-Soil (Horticulture) in 200 ml and 400 ml water.
Plastic pots (13.5 cm top diameter and 12 cm high) were partly filled with 1000 g sandy soils. On 8 October 2001, field planting of Pinus patula and Eucalyptus clonal seedlings raised in standard nursery containers was simulated in these pots by applying the above-mentioned Aqua-Soil and water quantities plus 600 g of the same sandy soil per pot.

For Pinus patula, each of the above-mentioned eight treatments was replicated six times.

For Eucalyptus the 5 g Aqua-Soil (Forest) in 500 ml water was omitted and the other seven treatments could only be replicated four times because of the limited number of available plants. The design was a complete randomised block.

The potted plants were placed in the Department of Forest Science nursery. All plants were irrigated daily with the same quantity of water, which varied between days because of differences in daily temperatures. Weeds were removed regularly by hand. No additional fertilizer was applied.

Shoot length was measured immediately after planting and again on 16 January 2002, i.e. 100 days after planting, when the trial was terminated. Representative plants of treatments were photographed. Roots of all plants were gently washed free of soil. For each plant the oven dry (80°C) mass of the roots that grew out of the original root plug, i.e. the root growth during the final period, as well as that of the shoot, were determined.

Shoot and root growth data were subjected to analyses of variance and the least significant differences (LSD) between treatment means were calculated.

 
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RESULTS

 
Plants that had been planted with Aqua-Soil were considerably greener than the control plants. 
Differences between different Aqua-Soil treatments were relatively small with the trend that the more diluted treatments were less effective. 
Plant survival was 100% in all treatments. 

The results are summarized in the following table, and also expressed as a percentage of the control, which was taken as 100% for every comparison.

 

Results of Aqua-Soil Pot Trial After 100 Days

 

Mean Height Increment

Mean Shoot Biomass

Mean Root Biomass

Treatment

cm

% of Control

g

% of
Control

g

% of
Control

Pinus patula

 

 

 

 

 

 

Control (400 ml water)

6,7

100

1,60

100

1,26

100

5 g Forest in 200 ml water

16,1

240

5,35

334

3,07

244

5 g Forest in 300 ml water

13,5

201

4,85

303

3,09

245

5 g Forest in 400 ml water

10,0

149

4,35

272

2,52

200

5 g Forest in 500 ml water

15,5

231

4,07

254

2,59

206

3 g Hort in 200 ml water

12,9

193

5,53

346

2,64

210

3 g Hort in 400 ml water

14,6

218

4,38

274

2,52

200

10 g Forest in 300 ml water

15,2

227

5,62

351

2,77

220

Eucalyptus clone

 

 

 

 

 

 

Control (400 ml water)

5,8

100

1,88

100

0,69

100

5 g Forest in 200 ml water

16,5

284

9,08

483

3,89

564

5 g Forest in 300 ml water

18,6

321

10,28

547

2,67

387

5 g Forest in 400 ml water

18,8

324

9,90

527

3,29

477

3 g Hort in 200 ml water

21,4

369

11,03

587

3,47

503

3 g Hort in 400 ml water

19,0

328

8,70

463

3,12

452

10 g Forest in 300 ml water

19,8

341

14,10

750

5,74

832

 
For Pinus patula mean height increment of all treatments except 5 g Aqua-Soil (Forest) in 400 ml water, was significantly (99% level) more than that of the control. Mean dry shoot biomass of all except one treatment (5 g Aqua-Soil (Forest) in 500 ml water) was significantly bigger than the control at the 99,9% level. The mean of the abovementioned treatment was significantly bigger than the control at the 99% level. The mean biomass of the oven dry root growth of all treatments with Aqua-Soil were significantly bigger than that of the control at the 95% level.

Mean differences between Aqua-Soil treated plants did not differ significantly.

For the Eucalyptus clone, mean height increments of all Aqua-Soil treatments were very highly significantly (at the 99.9% level) better than that of the control. Mean shoot biomass of all Aqua-Soil treatments were significantly (at the 99% level) more that that of the control.

 The mean height of plants treated with 10 g Aqua-Soil (Forest) was significantly (at the 95% level) bigger that the mean heights of all 5 g Aqua-Soil (Forest) and 3 g Aqua-Soil (Horticulture) in 200 ml water. Dry root growth biomass of plants treated with 10 g Aqua-Soil (Forest) was significantly more than that of all other Aqua-Soil treatments (at the 99% level).

Those treatments again were significantly more than that of the control, also at the 99% level.

 
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DISCUSSION

 
Aqua-Soil applied as a gel, generally significantly improved shoot and root growth of Pinus patula, and especially of Eucalyptus.

The pots were too small to simulate the standard fertilization practice at time of planting, i.e. 100 to 200 g granular fertilizer applied 20 to 30 cm from the plant.

The application of 10 g Aqua-Soil, i.e. double the recommended dosage, did not have a detrimental effect on the plants.

Although initially there were no visible differences in response of plants to the 5 g and 10 g Aqua-Soil, the heavier application eventually resulted in greener Pinus patula foliage, and significantly more Eucalyptus shoot and root mass. This may be due to the additional nutrients or the additional super-absorbant of a combination of both. This should be determined in another trial. An Aqua-Soil treatment that stimulates transplant growth throughout the first growing season, and not only for the first few months, is preferable, as quicker canopy closure will occur. Consequently weeding costs should be reduced.

 
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Pot trial with Aqua-Soil applied to Pinus Patula and a Eucalyptus clone
Nitrogen Retention Test
Phosphate Retention Test
Potassium Retention Test
Water Retention Test after 30 Days
 
Research Archive
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